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KMID : 0545120110210050515
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 5 p.515 ~ p.518
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Cloning and Expression of a bpr Gene Encoding Bacillopeptidase F from Bacillus amyloliquefaciens CH86-1
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Kwon Gun-Hee
Park Jae-Yong
Kim Jong-Sang
Lim Jin-Kyu
Park Cheon-Seok
Kwon Dae-Young
Kim Jeong-Hwan
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Abstract
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A gene encoding bacillopeptidase F, bpr86-1, was cloned from B. amyloliquefaciens CH86-1 isolated from cheonggukjang. This gene could encode a preproenzyme of 1,431 amino acids. When bpr86-1 was introduced into B. subtilis WB600 via pHY300PLK, an E. coli-Bacillus shuttle vector, the transformant showed fibrinolytic activity. During growth on LB, the fibrinolytic activity of cells increased sharply when they entered the stationary phase. The highest activity (761.4 mU/mg protein) was observed at 96 h of cultivation.
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KEYWORD
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Bacillus amyloliquefaciens CH86-1, bacillopeptidase F, bpr gene, fibrinolytic enzymes
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